What is Gluten Hydration?
Gluten hydration is an essential step in baking because it helps activate wheat gluten proteins.
In water, gliadin and glutenin proteins interact to establish bonds and form small protein strands. This is the basis for how a cohesive viscoelastic gluten matrix is developed. It’s essential for retaining gases during fermentation.1,2 A properly hydrated gluten network will:
- Allow the dough to be more extensible, stretchable and elastic
- Allow for a better sheeting and moulding of dough
- Improve cell structure of finished product
Gluten hydration provides a way for water molecules to bind to several polar groups in proteins:2
- Charged groups (ion–dipole interactions)
- Amide groups of asparagine and glutamine
- Hydroxyl groups of glutamine, serine, threonine, and tyrosine residues (hydrogen bonding)
Amino acids such as glutamine, threonine and serine are responsible for the water binding capacity of gluten. This is because around 30% of gluten is hydrophobic.2
When hydrated, gliadin is viscous and can be stretched to a thin strand that flows easily with gravity like glue. On the other hand, hydrated glutenin is very elastic and has a large resistance to deformation and stretching forces. When these two proteins are combined, they form a three-dimensional, continuous network called gluten. So, gluten has both viscous and elastic properties.3
Hydration impacts several functions and properties of gluten proteins:4
- Dough formation
- Gel formation
- Swelling behavior
- Thickening power
- Glass transition
- Water absorption / water-holding capacity
It is impossible to create dough with optimum handling properties without hydrating gluten proteins first. If the dough is not sufficiently hydrated, wheat flour proteins can’t participate in forming a network during baking.
Gluten hydration is influenced by:
- Temperature: the higher the temperature, the faster the hydration process.
- Amount of water: gluten proteins are not the only component of flour that take up water. So, sufficient water is needed to thoroughly hydrate all the gluten present.
- Mixing time: gluten proteins take time to fully hydrate as water gradually diffuses from wet zones to dry zones in the flour.
- Mixing intensity or mechanical work: mixing generates motion in particles. Also, it allows direct contact between flour particles and water.
- Flour particle size: the smaller the flour particle size, the larger the surface area exposed and the faster the hydration of gluten.
- Competition for water absorption: Flour components such as starch, arabinoxylans, β-glucans and cellulose also take up water. As a consequence, they compete with gluten for hydration. If the formula is not well balanced, then gluten will only be partially hydrated.
To see if the dough is properly hydrated and mixed, perform the gluten film or window pane test. A small portion of dough is stretched between the hands into a thin, smooth, translucent film to test its extensibility and elasticity:
Predicting the amount of water for gluten hydration
For example, say there is 100 g flour with a protein content of 13% (gluten accounts for 80%). Gluten needs to absorb about 2 times its own weight to become fully functional (2 g water / 1 g dry gluten).3
The question is, how much of the total water is used to hydrate gluten?
g dry gluten = 100 × 0.13 × 0.8 = 10.4
g water absorbed by gluten = 10.4 × 2 = 20.8
% water = (20.8 ÷ total absorption %) x 100
AACCI Methods used to assess gluten hydration properties
- 56-61.02 – Sedimentation test for wheat
- 56-11.02 – Solvent retention capacity profile (SRC)
- 38-12.02 – Glutomatic (wet/gluten gluten, water-binding capacity and gluten index)
- 56-30.01 – Water hydration capacity of protein materials
- Wrigley, C.W., Békés, F., Bushuk, W. “Gluten: A Balance of Gliadin and Glutenin.” Gliadin and Glutenin: The Unique Balance of Wheat Quality, AACC International, Inc., 2006, pp. 3–32.
- Damodaran, S. “Amino Acids, Peptides, and Proteins.” Fennema’s Food Chemistry, 5th Edition, CRC Press, Taylor & Francis Group, LLC, 2017, pp. 235–351.
- Finnie, S., and Atwell, W.A. “Composition of Commercial Flour.” Wheat Flour, 2nd Edition, AACC International, Inc., 2016, pp. 31–42.
- Li-Chan, E.C.Y., and Lacroix, I.M.E. “Properties of Proteins in Food Systems: An Introduction.” Proteins in Food Processing, 2nd Edition, Woodhead Publishing, Elsevier Inc., 2018, pp. 19–45.
I add vital wheat gluten to my bread flour to bring the protein content up to 14.5%. I also utilize a poolish, in my case, 264 grams of bread flour, with 12.7% protein, plus 8 grams of VWG. How much water should I use in this poolish? I’m thinking 264 grams of water to cover the bread flour, but more than 8 grams of water to cover the VWG, because it absorbs more water. Is there a recommended amount of “extra” water to add to flour that includes VWG? Thank you. Great service.
hi Eugene, we are addressing questions in our forum, so others can participate and contribute. Please post your questions here: https://bakerpedia.com/forums/ Thank you!
% water = (20.8 ÷ 62) x 100 = 33.5%
Thank you for very interesting material. Could please give an explanation where 62 number comes in this formula ?
Great question Corin. It’s the total absorption, which is usually between 60-65% for most bakeries. We’ve updated it to the term, so it makes more sense.