lipase enzyme

Lipase

Also known as triacylglycerol acyl-hydrolase, galactolipase or phospholipase


What is lipase?

Lipase is one type of hydrolytic enzymes responsible for degrading or modifying nonpolar (e.g. triglycerides) and polar lipids (e.g. galactolipids and phospholipids) found in both animal and plant sources.

Lipase is a clean label ingredient, considered as a processing aid.

  • It can partially or totally replace traditional dough strengthening emulsifiers, such as anionic surfactants DATEM and SSL.
  • Lipase can also replace crumb softening products such as glyceryl monostearate (GMS).

Origin

Lipases are key enzymes involved in fat digestion in humans and animals by converting insoluble triacylglycerols into more soluble products, fatty acids and mono-acylglycerols, that can easily be metabolized for energy generation.1

It can be found in many plants and animals. In recent decades, lipases have been produced commercially from highly specialized microorganisms such as bacteria, yeast and fungi.

Function

Lipases catalyze hydrolytic reactions of lipids by cleaving the carboxylic ester bonds between the glycerol backbone and one or more of the fatty acid chains.

The term ’lipase’ is usually expressed as a singular noun although it encompasses different types of lipolytic enzymes. The following table lists lipases which perform essential functions in baked goods, such as foam emulsification (i.e. gas bubble stabilization) and crumb softening through starch complexing.

Lipases act on the endogenous lipids found in wheat flour and other lipids added in the form of oil or lecithin. Flour lipids are a complicated mixture of compounds. Of the 2.0–3.0% (w/w) lipid material found in whole wheat flour, about 50% are non-polar lipids (triacyl glycerides, fatty acids, sterols, and their esters), and the other half are strongly polar (phospholipids and galactolipids).2

Lipase EC number Substrate Reaction catalyzed Ingredient replaced
Triacylglycerol lipase 3.1.1.3 Non-polar lipids in vegetable fats and oils Triacylglycerol (TAG) + H2O →

Diacylglycerol (DAG) + Free fatty acid (FFA)

Diacylglycerol (DAG) + H2O →

Monoacylglycerol (MAG) + FFA

GMS

Distilled

monoglycerides

Phospholipase A1 3.1.1.32 Polar lipids found in cereal flours and soy/sunflower lecithin Phosphatidyl-choline (PC) + H2O → Lysophosphatidyl-choline (LPC) + FFA

Phosphatidyl-ethanolamine (PE) + H2O → Lysophosphatidyl-ethanolamine (LPE) + FFA

N-acyl-phosphatidyl-ethanolamine (NAPE) + H2O → N-acyl-lysophosphatidyl-ethanolamine (NALPE) + FFA

DATEM, SSL, CSL

Egg reduction / egg replacement

Phospholipase A2 3.1.1.4 Polar lipids found in cereal flours and soy/sunflower lecithin DATEM, SSL, CSL

Egg reduction / egg replacement

Galactolipase 3.1.1.26 Polar lipids found in cereal flours and soy/sunflower lecithin Digalactosyl-diglyceride (DGDG) + H2O → Digalactosyl-monoglyceride (DGMG) + FFA DATEM, SSL, CSL

Commercial production

Despite the prevalence of lipase enzymes in cereals and oil seeds, large scale production relies on fermentation and downstream processing using modern biotechnology.

The following block diagram summarizes the commercial production of lipase:

A block diagram that summarizes the commercial production of lipase.

Application

Lipases, as stand alone ingredients, do not replace emulsifiers. They need proper conditions to function and a sufficient amount of substrate to render the desired effect on the dough or batter. For dough strengthening purposes and foam emulsification, the key aspect about lipases is that they transform polar lipids into functional lysolipids which have superior surface activity, higher HLB value and a greater capacity to absorb at oil/water or/and gas/liquid interfaces.3,4

Polar lipids in flour + Lipase + Water → Lysolipids (e.g. LPC, NALPE, LPE, DGMG) (strong emulsifiers)

Like  other enzymes, lipases require special conditions for optimum activity and performance, namely:

  • Acidity (pH): Most lipases prefer rather alkaline conditions (i.e. a pH of 8.0), so higher doses are needed to obtain equivalent functionality.
  • Temperature: Most lipases prefer temperatures above 45°C (113°F). Chemical reaction rates double for every 10°C (18°F) increase in temperature.
  • Enzyme-substrate contact time.
  • Sufficient water activity to support chemical reaction.
  • Amount of substrate (enzymes work better when there is excess substrate).
  • Adequate enzyme dosage (excess FFA may result in a rancid taste). Lipase overdose could also lead to a breakdown of the in-situ produced lysolipids, increasing the risk of gas bubble coalescence and rupture, and eventually a loss of oven spring.

Regulation

Similar to  other enzymes, food lipases are GRAS (Generally Recognized as Safe) and often considered a food processing aid in the US. The FDA regulates their origin (food-compatible) and establishes limits to their use (if applicable) based on GMP.5

References

  1. Wong, D.W.S. “Lipase.” Handbook of Food Enzymology, Marcel Dekker, Inc., 2003, pp. 653–665.
  2. MacRitchie, F. Lipids in Cereal Technology, Academic Press Inc., 1983.
  3. Putseys, J.A., and Schooneveld-Bergmans, M. “Enzymes Used in Baking.” Industrial Enzyme Applications, Wiley-VCH Verlag GmbH & Co. KGaA, 2019, pp. 97–123.
  4. Van Oort, M. “Enzymes in Bread Making.” Enzymes in Food Technology, 2nd edition, Blackwell Publishing Ltd, 2010, pp. 103–143.
  5. Smith, J. “Enzymes.” Food Additives Data Book, 2nd edition, Blackwell Publishing Ltd., 2011, pp. 366–454.

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