Improve Your Dough Improver with Transglutaminase Enzyme

Croissant flaky dough transglutaminase

It is no secret that, if used properly, the application of the transglutaminase enzyme (TG) in bakery products can promote great improvement in dough texture, volume, and gas retention, even when combined with other enzymes.

Transglutaminases (EC 2.3.2.13) γ-glutamyl-peptide, amine-γ-glutamyl-transferase) belong to the group of acyltransferases, which catalyze acyl-transfer reactions between a γ-carboxyamine, group of a peptide – or protein-bond glutamyl residue and a primary amino group of various substrates including the ε-amino group of lysine or lysyl residues in proteins, resulting in polymerization or amine incorporation.

The result cross-link is called a ε-(γ-glutamyl)lysine isopeptide bond). During the reaction a single molecule of ammonia is generated per cross-link. In case the amine substrates are not available as acyl acceptors, it is possible for TG to catalyse deamination of glutamyl residues using water as an acyl acceptor. In contrast to their limited glutamine (an acyl donor) substrate specificity, TG possesses a wide specificity for the acyl acceptor substrates (Fig. 1).

Figure 1 – Transglutaminase mode of action. a) Acyl transfer, b) Crosslinking of Gln and Lys residues in proteins or peptides, c) Deamidation.

Figure 1 – Transglutaminase mode of action. a) Acyl transfer, b) Crosslinking of Gln and Lys residues in proteins or peptides, c) Deamidation.

Transglutaminase applications and effects:

Application Effect Reference
Pastry product Improved preservation and lift of puffy pastry Gerrard et al, 2000
Frozen, laminated doughs Improved stability, texture and volume Gerrard 2002, Pozza 2002
Low quality flour baking Rebuilding the structure of dough Koksel et al 2001, Cabellero et al, 2005
Noodles and pasta manufacturing Improved texture Wu and Cork, 2005
Gluten-free baking Formation of protein network, improved loaf volume, crumb Moore et al, 2006
Frozen dough Stabilize the gluten structure embedded by starch granules Huang et al, 2008
Rice noodles Cooking loss and water turbidity of rice noodle decreased Kim et al, 2013
Dough improvers Improved volume, texture and shelf life of baked goods Esteller, 2016
Fig. 2 – TG in croissant (from J.A. Gerrard et al., JFS, v. 65, n. 2, 2000).

Fig. 2 – TG in croissant (from J.A. Gerrard et al., JFS, v. 65, n. 2, 2000).

Fig. 3 – Addition of 0.03%/300g wheat flour with concentrated and encapsulated transglutaminase corresponding to 25-35 u/g (Mauricio Esteller, 2016).

Fig. 3 – Addition of 0.03%/300g wheat flour with concentrated and encapsulated transglutaminase corresponding to 25-35 u/g (Mauricio Esteller, 2016).

So what’s new?

What few people know, however, is the deleterious effects of oxygen (it can oxidize sulfhydryl groups) on the stability of the transglutaminase enzyme and the consequent reduction or loss in activity during storage and manipulation.

Most well-known manufacturers tend to store TG in vacuum packages and refrigerated storage, which causes many setbacks after the package is opened. It needs to be fractionated over successive applications if vacuum pumps or barrier films are not available.

How to solve the problem?

By using an innovative technology which concentrates and encapsulates the protein this issue can be avoided without affecting performance of the transglutaminase enzyme in industrial processing or long storage of the dough improver. New trials with this new stable TG generation showed best improved effects.

2018-12-12T11:15:41+00:00

About the Author:

Mauricio Esteller
Mauricio Esteller is a relentless "Enzymesaur," who has been working as a Enzymes Specialist for more than 30 years in Food and non Food Industry. He specializes in the improvement of enzymes performance and new applications. He wants to know, "Can we biotransform it? I'm interested!"

One Comment

  1. RoyBasan March 1, 2018 at 4:40 pm - Reply

    Does it mean that you can’t use TG with conventional dough oxidants like ascorbic acid, glucose oxidase so as to create a multifunctional clean label dough improver..?

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